CST, 86050S, TIA-1 (D1Q3K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIA1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TIA-1 (D1Q3K) Rabbit Monoclonal Antibody (CST #86050) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TIA-1 (D1Q3K) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIA-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIA-1 protein. Background T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, Cox2, cytochrome c, GADD45α, and HIF-1α (8-13). Alternate Names Cytotoxic granule associated RNA binding protein TIA1; cytotoxic granule-associated RNA-binding protein; nucleolysin TIA-1; Nucleolysin TIA-1 isoform p15; Nucleolysin TIA-1 isoform p40; p15-TIA-1; p40-TIA-1; p40-TIA-1 (containing p15-TIA-1); RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; TIA1; TIA1 cytotoxic granule associated RNA binding protein; TIA1 cytotoxic granule-associated RNA binding protein; TIA1 cytotoxic granule-associated RNA-binding protein; WDM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42, 44 Source/Isotype: Rabbit IgG