CST, 86051T, Iduronate 2-Sulfatase (F5F9E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IDS. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Iduronate 2-Sulfatase (F5F9E) Rabbit Monoclonal Antibody (CST #86051) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Iduronate 2-Sulfatase (F5F9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total iduronate 2-sulfatase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro160 of human iduronate 2-sulfatase protein. Background Iduronate 2-sulfatase (IDS) is a lysosomal enzyme expressed in pancreatic islets responsible for proteoglycan degradation, such as dermatan sulfate and heparan sulfate. It has a role in glucose-stimulated insulin secretion via a mechanism involving exocytosis activation through phosphorylation of protein kinase C alpha (PKCα) and its Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) (1). IDS was shown to be glycosylated in the endoplasmic reticulum (ER) and Golgi apparatus and proteolytically cleaved to generate the mature forms in the Golgi apparatus. Mature IDS is then translocated to the lysosome (2). Mutations in the IDS gene cause the progressive disease mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, an X-linked recessive lysosomal storage disorder (2-4). Inactivity, insufficiency, or absence of IDS leads to widespread accumulation of proteoglycans and subsequent lysosomal dysfunction in multiple organs and tissues, including the central nervous system (CNS) (4). Alternate Names Alpha-L-iduronate sulfate sulfatase; ID2S; IDS; Iduronate 2-sulfatase; Iduronate 2-sulfatase 14 kDa chain; Iduronate 2-sulfatase 42 kDa chain; iduronate-2-sulfatase; Idursulfase; MPS2; SIDS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG