CST, 8605T, SINTBAD (D1A5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SINTBAD. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SINTBAD (D1A5) Rabbit Monoclonal Antibody (CST #8605) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SINTBAD (D1A5) Rabbit Monoclonal Antibody recognizes endogenous levels of total SINTBAD protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu55 of human SINTBAD protein. Background SINTBAD, identified based on homology to NAP1, is an adaptor protein for TBK1 and IKKε, two related kinases that play a pivotal role in innate immune response (1). These kinases trigger the phosphorylation and subsequent activation of the transcription factor IRF-3, resulting in gene regulation required for immune responses (2). The precise mechanism by which SINTBAD activates TBK1 and IKKε is still unclear. Expression of a central region of SINTBAD termed a TBD (TBK binding domain) can function as a dominant negative protein and interfere with IRF-3 activation. SINTBAD and NAP1 can also bind to NDP52, a protein that associates with poly-ubiquitinated proteins, such as those produced on the surfaces of bacteria, and can trigger autophagy (3). Alternate Names KIAA0775; ProSAPiP2; SINTBAD; TANK-binding kinase 1-binding protein 1; TBK1 binding protein 1; TBK1-binding protein 1; TBKB1; TBKBP1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78-80 Source/Isotype: Rabbit IgG