CST, 86100SF, Neurofilament-L (E3V7Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NFL. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Neurofilament-L (E3V7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86100) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Neurofilament-L (E3V7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Neurofilament-L protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminal region of human Neurofilament-L. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; FLJ53642; light molecular weight neurofilament protein; NEFL; Neurofilament L; neurofilament light; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament-light; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; NF-L; NF68; NFL; PPP1R110; protein phosphatase 1, regulatory subunit 110 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG