CST, 8611S, TIAR (D26E4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIAR. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, TIAR (D26E4) Rabbit Monoclonal Antibody (CST #8611) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TIAR (D26E4) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIAR protein. This antibody also weakly recognizes recombinant TIA1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro337 of human TIAR protein. Background TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6). Alternate Names aging-associated gene 7 protein; MGC33401; Nucleolysin TIAR; T-cluster binding protein; TCBP; TIA-1 related protein; TIA-1-related nucleolysin; TIA-1-related protein; TIA1 cytotoxic granule associated RNA binding protein like 1; TIA1 cytotoxic granule-associated RNA binding protein-like 1; TIA1 cytotoxic granule-associated RNA-binding protein-like 1; TIA1 related; TIAL1; TIAR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG