CST, 8612S, CA2 Antibody

Polyclonal Antibody for studying CA2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CA2 Antibody (CST #8612) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CA2 Antibody recognizes endogenous levels of total CA2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys169 of human CA2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO into HCO and H and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2). CA2 is a cytosolic member of the α class. It is the most widely distributed isoform among the mammalian CAs (1). Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (3-5). Elevated expression of CA2 is observed in patients with Alzheimer's disease and the developing brains of Down syndrome patients (6,7). CA2 is also overexpressed in Gastrointestinal Stromal Tumors (GISTs) and is considered a useful marker for diagnosis (8). Recently, CA2 was reported to facilitate transporter activity of the monocarboxylate transporter isoform 1 and 4 (MCT1/4) independent of its own catalytic activity (9,10) Alternate Names CA-II; CA2; CAC; CAH2; CAII; Car2; Carbonate dehydratase II; Carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; carbonic dehydratase; epididymis luminal protein 76; epididymis secretory protein Li 282; HEL-76; HEL-S-282 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit