CST, 86158T, CD59 (E8A4S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD59. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD59 (E8A4S) Rabbit Monoclonal Antibody (CST #86158) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD59 (E8A4S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD59 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln99 of human CD59 protein. Background CD59 is a GPI-anchored membrane protein that functions as an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes, therefore inhibiting the complement-dependent cytolysis (CDC) (1). CD59 is a ubiquitously expressed cell membrane protein that protects cells from CDC. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients (2,3). Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Potent inhibitors for CD59 have been actively pursued for therapeutic applications (4,5). In addition, CD59 may regulate insulin secretion by modulating exocytosis (6). Alternate Names 16.3A5; 1F5; 1F5 antigen; 20 kDa homologous restriction factor; CD59; CD59 antigen; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 blood group antigen; CD59 glycoprotein; CD59 molecule (CD59 blood group); CD59 molecule, complement regulatory protein; EJ16; EJ30; EL32; FLJ38134; FLJ92039; G344; HRF-20; HRF20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MGC2354; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG