CST, 86188SF, INPP4b (D9K1B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying INPP4B. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, INPP4b (D9K1B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86188) is ready to ship. Product Usage Information This product is the carrier free version of product #14543. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14543 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity INPP4b (D9K1B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total INPP4b protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn773 of human INPP4b protein. Background Phosphatidylinositol lipids and phosphoinositides are important second messengers, their generation controlling many cellular events. Intracellular levels of these molecules are regulated by phosphoinositide kinases and phosphatases. One of the best characterized lipid kinases is phosphoinositide 3-kinase (PI3K), which is responsible for phosphorylation on the D-3 position of the inositide head group (1). This action of PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN, the well characterized partnering phosphatase, reverses this process by removing the phosphate from PI(3,4,5)P3 at the D-3 position to generate PI(4,5)P2 (1,2). Dephosphorylation on the D-5 position to generate PI(3,4)P2 occurs through the action of SHIP1 or SHIP2 (3), and dephosphorylation on the D-4 position to generate PI(3)P can occur through the action of inositol polyphosphate 4-phosphatase isoenzymes type I (INPP4a) and type II (INPP4b) (4,5). While INPP4a has been implicated in neuronal survival and megakaryocyte lineage determination (6,7), less is understood about INPP4b. It has been shown that two splice variants of INPP4b occur in mice, each showing distinct tissue distribution and subcellular localization (5,8). Alternate Names 4-phosphatase II; inositol polyphosphate 4-phosphatase II; Inositol polyphosphate 4-phosphatase type II; inositol polyphosphate-4-phosphatase type II B; inositol polyphosphate-4-phosphatase, type II, 105kDa; INP4B; INPP4B; MGC132014; Type II inositol 3,4-bisphosphate 4-phosphatase; Type II inositol-3,4-bisphosphate 4-phosphatase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG