CST, 86225T, Rad50 (E3I8K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rad50. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Rad50 (E3I8K) Rabbit Monoclonal Antibody (CST #86225) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Rad50 (E3I8K) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rad50 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Rad50 protein. Background The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes (SMC) family and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and NBS1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2). ATM-dependent phosphorylation of Rad50 at Ser635 in response to DNA damage is important in regulating downstream signaling, DNA repair, and checkpoint control (5). Alternate Names DNA repair protein RAD50; hRAD50; NBSLD; RAD50; RAD50 double strand break repair protein; RAD50 homolog (S. cerevisiae); RAD50 homolog, double strand break repair protein; RAD50-2; RAD502 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 153 Source/Isotype: Rabbit IgG