CST, 86236SF, MAFA (D2Z6N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAFA. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, MAFA (D2Z6N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86236) is ready to ship. Product Usage Information This product is the carrier free version of product #79737. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79737 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MAFA (D2Z6N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MAFA protein. Based on sequence similarity, this antibody is not predicted to cross-react with MAFB or c-MAF. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala345 of human MAFA protein. Background MAFA belongs to the musculoaponeurotic fibrosarcoma (MAF) family of basic leucine-zipper transcription factors (1). In the mouse embryo, MAFA expression is first detected at E13.5, restricted to Nkx6.1-positive insulin-producing islet cells (2). Expression of the gene is sensitive to physiological glucose levels, and genomic targets regulated by MAFA include β-cell transcription factors (e.g., ) and the insulin gene (2, 3). Ectopic expression of MAFA was shown to induce insulin production by pancreatic α-cells (2), while conditional overexpression of MAFA promoted transdifferentiation of α-cells into insulin-producing β-cells (4). Targeted deletion of the gene in mice likewise led to a loss of β-cell identity and function (5). Collectively, these data suggest that MAFA is critical for the development, maintenance, and physiological function of insulin-producing pancreatic β-cells, highlighting its potential utility as a target for translational and clinical research studies in diabetes (6). Alternate Names hMafA; INSDM; MAF bZIP transcription factor A; MAFA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1; RIPE3b1 factor; Transcription factor MafA; Transcription factor RIPE3b1; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG