CST, 86241S, GATA-3 (D13C9) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying GATA3. Validated for Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, GATA-3 (D13C9) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #86241) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:1000 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity GATA-3 (D13C9) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr63 of human GATA-3 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 null mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12). Alternate Names GATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG