CST, 86302S, FAM96B (D4V9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAM96B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FAM96B (D4V9Z) Rabbit Monoclonal Antibody (CST #86302) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FAM96B (D4V9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAM96B protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln21 of human FAM96B protein. Background Iron-sulfur (Fe-S) clusters (ISC) are cofactors for many proteins that display a wide range of biological functions, such as DNA maintenance, transcription, translation, cellular metabolism, electron transport, and oxidative phosphorylation (1). While structurally simple, the synthesis and insertion of ISC into Fe-S proteins are complex processes that involve many different proteins (2). FAM96B, also known as MIP18 (MSS19-interacting protein of 18kDa), is a component of the cytosolic Fe-S protein assembly complex (3,4). FAM96B is in a complex with MMS19 that is responsible for assembly of multiple nuclear Fe-S proteins involved in DNA metabolism (5). Alternate Names CGI-128; CGI-128 protein; CIA2B; CIAB; CIAO2B; Cytosolic iron-sulfur assembly component 2B; FAM96B; family with sequence similarity 96 member B; family with sequence similarity 96, member B; MIP18; Mitotic spindle-associated MMXD complex subunit MIP18; MSS19-interacting protein of 18 kDa; Protein FAM96B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG