CST, 86326S, SARS-CoV-2 Nucleocapsid Protein (E9L7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Nucleocapsid Protein SARS-CoV-2. Validated for WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 Nucleocapsid Protein (E9L7H) Rabbit Monoclonal Antibody (CST #86326) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SARS-CoV-2 Nucleocapsid Protein (E9L7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 nucleocapsid (N) protein. This antibody has been confirmed to detect the following SARS-CoV-2 isolates and variants: USA-WA1/2020, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (B.1.1.529). This antibody does not cross-react with nucleocapsid (N) protein from SARS-CoV-1 coronavirus. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of SARS-CoV-2 nucleocapsid (N) protein (USA-WA1/2020 isolate). Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7). Alternate Names 2019-nCoV nucleocapsid protein; NC; NCAP_SARS2; nucleocapsid phosphoprotein; Nucleocapsid protein; Nucleoprotein; Protein N; SARS-CoV-2 nucleocapsid; Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG