CST, 8635S, IkappaB beta (7B4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IkB-beta. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IkappaB beta (7B4) Mouse Monoclonal Antibody (CST #8635) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IkappaB beta (7B4) Mouse Monoclonal Antibody recognizes endogenous levels of total IκBβ protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a carboxy terminal fragment of human IκBβ protein. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). The regulation of IκBβ and IκBε is similar to that of IκBα. However, the phosphorylation and ubiquitin-mediated degradation of these proteins occurs with much slower kinetics (9). IKK phosphorylation of IκBβ occurs at Ser19 and Ser23, while IκBε can be phosphorylated at Ser18 and Ser22 (10). Alternate Names I-kappa-B-beta; IkappaBbeta; IkB-B; IkB-beta; IKBB; NF-kappa-B inhibitor beta; NF-kappa-BIB; NFKB inhibitor beta; NFKBIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9; TRIP9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Mouse IgG1