CST, 8636S, LASP1 Antibody

Polyclonal Antibody for studying Lasp1. Validated for Western Blotting. Highly specific and rigorously validated in-house, LASP1 Antibody (CST #8636) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LASP1 Antibody detects endogeneous levels of total LASP1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala191 of human LASP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background LASP1 is a cytoskeletal scaffold protein belonging to the LIM protein subfamily (1,2). LASP1 consists of an N-terminal LIM domain, followed by two nebulin repeats, and a C-terminal SH3 domain (1,3). The nebulin repeats interact with actin, while the SH3 domain interacts with palladin (4,5), suggesting LASP1 functions as an actin-binding protein, possibly in cytoskeletal organization. LASP1 has been shown to localize to focal adhesions, lamellipodia, and membrane ruffles (6-8) and might be involved in membrane migration. Overexpression of LASP1 has been associated with metastatic cancers, such as breast and ovarian cancer (2). In these cases, membrane, cytoplasmic, and nuclear localization of LASP1 in the tumor cell has been reported, suggesting LASP1 involvement in membrane and nuclear signaling (9,10). Alternate Names LASP-1; LASP1; LIM and SH3 domain protein 1; LIM and SH3 protein 1; Metastatic lymph node gene 50 protein; MLN 50; MLN50 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit