CST, 86377T, p300 (D8Z4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p300. Validated for WB,IP,IHC,IF,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, p300 (D8Z4E) Rabbit Monoclonal Antibody (CST #86377) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18396 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), CUT&RUN, CUT&Tag Specificity / Sensitivity p300 (D8Z4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total p300 protein. This antibody does not cross-react with CBP protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p300 protein. Background CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9). Alternate Names E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; EP300; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; KAT3B; MKHK2; P300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein propionyltransferase p300; RSTS2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG