CST, 8637T, RRM1 (D12F12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RRM1. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, RRM1 (D12F12) Rabbit Monoclonal Antibody (CST #8637) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63356 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity RRM1 (D12F12) Rabbit Monoclonal Antibody recognizes endogenous levels of total RRM1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RRM1 protein. Background Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4). Alternate Names R1; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; ribonucleotide reductase catalytic subunit M1; Ribonucleotide reductase large subunit; ribonucleotide reductase M1; ribonucleotide reductase M1 polypeptide; ribonucleotide reductase, large subunit; ribonucleotide reductase, R1 subunit; RIR1; RR1; RRM1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit