CST, 86425T, RBM5 (E8G8K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBM5. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM5 (E8G8K) Rabbit Monoclonal Antibody (CST #86425) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RBM5 (E8G8K) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM5 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly325 of human RBM5 protein. Background RBM5 is an RNA binding protein that regulates alternative splicing through association with the spliceosome (1,2). First identified as a tumor suppressor gene, RBM5 interacts with U2AF65 in order to regulate many pro-apoptotic genes, such as Fas (3). RBM5 is notably deleted in many lung cancers, and also has been shown to be a key regulator in male germ cell pre-mRNA splicing (4-6). Alternate Names FLJ39876; G15; H37; LUCA-15; LUCA15; Protein G15; Putative tumor suppressor LUCA15; RBM5; Renal carcinoma antigen NY-REN-9; RMB5; RNA binding motif protein 5; RNA-binding motif protein 5; RNA-binding protein 5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG