CST, 86426S, Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate)

Secondary Antibody for studying in the research area. Product Usage Information The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:400 - 1:1600 should yield acceptable results for immunofluorescent assays. When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration. FITC has relatively low photostability. Usage of an antifade reagent is recommended to preserve signal intensity. Storage Supplied lyophilized with a formulation of 0.02 M potassium phosphate, 0.15 M sodium chloride (pH 7.2), 10 mg/mL BSA, and 0.01% azide at 4ºC. Reconstitute lyophilized material with 1.0 mL dH2O and centrifuge until clear. The product is stable for six weeks at 4ºC as an undiluted liquid. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles. Specificity / Sensitivity Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate) reacts with the Fc portion of rabbit IgG. This antibody conjugate does not react with the Fab portion of rabbit IgG or human serum proteins. Species Reactivity: Rabbit Background This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence. Specification REACTIVITY: Rab Source/Isotype: Goat