CST, 86441SF, RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying AML2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86441) is ready to ship. Product Usage Information This product is the carrier free version of product #9647. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9647 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity RUNX3/AML2 (D6E2) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of both isoforms of RUNX3 protein. This antibody recognizes mouse RUNX3/AML2 protein and is also reactive with human RUNX3/AML2; however, this antibody is not suggested for immunohistochemical analysis of human tissues. This antibody does not recognize RUNX1 and RUNX2 proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RUNX3 protein. Background Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10). Alternate Names Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; AML2; CBF-alpha-3; CBFA3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; FLJ34510; MGC16070; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; PEBP2A3; PEBP2aC; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt related transcription factor 3; Runt-related transcription factor 3; RUNX family transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; transcription factor AML2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 43-48 Source/Isotype: Rabbit IgG