CST, 86513T, NID1/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Nidogen-1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, NID1/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody (CST #86513) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NID1/Nidogen-1 (E9J3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total NID1/nidogen-1 protein. This antibody cross-reacts with a band of unknown origin at approximately 80 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1186 of human NID1/nidogen-1 protein. Background All extracellular basement membrane contains four major components: type IV collagen, nidogens, laminins, and perlecan (1). Nidogens are a family of highly conserved, sulfated glycoproteins which interact with the laminin 1 short arm chain, type IV collagen, and perlecan. This linking effect contributes to basement membrane formation (2,3). There are two isoforms in the nidogen family, nidogen-1, and nidogen-2. They have a similar domain structure consisting of three globular domains separated by a link region and a rod region (3). The functions of the two isoforms are complementary. Knockout of both genes results in defective basement membrane formation and perinatal lethality due to abnormal lung/heart/limb development (4,5). In lung cancer, colorectal cancer, and ovarian cancer, increased nidogen-1 release contributes to tumor progression by promoting proliferation and migration/invasion, EMT, and metastasis (6-10). Alternate Names enactin; Entactin; epididymis secretory sperm binding protein; NID; NID-1; NID1; nidogen 1; Nidogen-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG