CST, 86519S, NPTXR (F8B3U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NPTXR. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NPTXR (F8B3U) Rabbit Monoclonal Antibody (CST #86519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NPTXR (F8B3U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NPTXR protein. This antibody does not cross-react with other NPTX proteins. This antibody detects a 20 kDa protein of unknown identity in some cell lines and tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NPTXR protein. Background The neuronal pentraxin (NPTX) family is composed of two secreted proteins, neuronal pentraxin 1 (NPTX1) and 2 (NPTX2), along with the NPTX receptor (NPTXR), a type II transmembrane protein. These proteins are part of the larger, evolutionarily conserved pentraxin superfamily. NPTX1, NPTX2, and NPTXR are known as long pentraxins. They share a conserved C-terminal domain with the short pentraxin family, which consists of C-reactive protein (CRP) and serum amyloid P component (SAP), but they differ in that they also have unique, long N-terminal sequences containing low complexity regions. NPTXs play vital roles in synaptogenesis, synaptic pruning and plasticity, debris clearance, and neurite outgrowth (1-4). NPTX1 and NPTX2 are primarily secreted at presynaptic terminals of excitatory neurons. Once secreted into the synaptic cleft, they form homo or hetero-oligomers via their C-terminal pentraxin domains and bind NPTXR on the postsynaptic membrane. This complex then acts to cluster α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), modulating synaptic activity and neuronal function (3,4). Synaptic dysfunction and neuronal loss are hallmarks of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and frontotemporal lobar degeneration (FTLD). Expression levels of NPTX family members in the brain, cerebrospinal fluid (CSF), and plasma have been shown to be altered in AD, PD, and FTLD, suggesting that they may be markers for this dysfunction, as well as diagnostic and therapeutic targets for these diseases (3,5-7). NPTX2 expression can also be regulated by TAR DNA-binding protein 43 (TDP43), suggesting a possible link to TDP43 dysfunction and related proteinopathies such as FTLD and amyotrophic lateral sclerosis (ALS) (8). Alternate Names Neuronal pentraxin receptor; NPR; NPTXR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40-65 Source/Isotype: Rabbit IgG