CST, 86533SF, AGR2 (D9V2F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying AGR2. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, AGR2 (D9V2F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86533) is ready to ship. Product Usage Information This product is the carrier free version of product #13062. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13062 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity AGR2 (D9V2F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total AGR2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala29 of human AGR2 protein. Background Anterior gradient homolog 2 (AGR2) is a member of the protein disulfide isomerase (PDI) family of proteins and a homolog of the cement gland protein (1). In normal human tissues, AGR2 is expressed most abundantly in intestinal cells. Research studies have found AGR2 is overexpressed in a number of adenocarcinomas, including those derived from breast, pancreas, ovary, prostate and esophagus (2-4). and studies have shown that AGR2 positively regulates cell growth and division, while its overexpression can promote cell transformation (5,6). The latter functions of AGR2 were shown to involve YAP1-mediated up-regulation of amphiregulin expression, implicating AGR2 in both the EGF and Hippo kinase signaling pathways (6). Alternate Names AG-2; AG2; AGR2; anterior gradient 2 homolog; anterior gradient 2, protein disulphide isomerase family member; anterior gradient homolog 2; anterior gradient homolog 2 (Xenopus laevis); Anterior gradient protein 2 homolog; epididymis secretory protein Li 116; GOB-4; hAG-2; HEL-S-116; HPC8; PDIA17; protein disulfide isomerase family A, member 17; secreted cement gland homolog; Secreted cement gland protein XAG-2 homolog; XAG-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG