CST, 8657S, PA28 alpha (D4F12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSME1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PA28 alpha (D4F12) Rabbit Monoclonal Antibody (CST #8657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PA28 alpha (D4F12) Rabbit Monoclonal Antibody recognizes endogenous levels of total PA28α protein. This antibody does not cross-react with either PA28β/PSME2 or PA28γ/PSME3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PA28α protein. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4). Alternate Names 11S regulator complex alpha subunit; 11S regulator complex subunit alpha; 29-kD MCP activator subunit; Activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; HEL-S-129m; IFI5111; IGUP I-5111; Interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; MGC8628; PA28a; PA28alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; proteasome activator subunit 1; proteasome activator subunit-1; PSME1; REG-alpha; REGalpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG