CST, 86590SF, Sall4 (D16H12) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SALL4. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Sall4 (D16H12) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86590) is ready to ship. Product Usage Information This product is the carrier free version of product #8459. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8459 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Sall4 (D16H12) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Sall4A and Sall4B proteins. Western blot analysis detects additional bands that are likely Sall4 isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala311 of human Sall4 protein. Background Members of the gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in have been implicated in human malformation syndromes, including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7). Alternate Names dJ1112F19.1; DRRS; HSAL4; MGC133050; sal-like 4 (Drosophila); Sal-like protein 4; SALL4; spalt like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80, 142 Source/Isotype: Rabbit IgG