CST, 86622S, LRBA Antibody

Polyclonal Antibody for studying LRBA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LRBA Antibody (CST #86622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LRBA Antibody recognizes endogenous levels of total LRBA protein. This antibody detects a 130 kDa band of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LRBA protein. Antibodies are purified by protein A and peptide affinity chromatography. Background LRBA (lipopolysaccharide responsive beige-like anchor protein) is a 300 kDa intracellular protein that is a member of the PH-BEACH-WD40 protein family (1-3). Its BEACH domain contains an A-kinase anchoring protein (AKAP) motif, known to have an affinity for signaling enzymes (1,3). LRBA has been found to be overexpressed in several different cancers, such as renal, pancreas, colorectal, and lung (2). LRBA deficiency, caused by various mutations, can cause common variable immune deficiency (CVID) as well as other immune dysregulations (3,5). LRBA plays an immunoregulatory role to CTLA-4, protecting it from being degraded within lysosomes (4,6). In LRBA deficiency, CTLA-4 is synthesized normally, but is not delivered to Rab GTPase recycling compartments, instead resulting in enhanced lysosome degradation (4,6,7). Alternate Names Beige-like protein; BGL; CDC4-like protein; CDC4L; cell division cycle 4-like; CVID8; DKFZp686A09128; DKFZp686K03100; DKFZp686P2258; FLJ16600; FLJ25686; LAB300; LBA; Lipopolysaccharide-responsive and beige-like anchor protein; LPS responsive beige-like anchor protein; LPS-responsive vesicle trafficking, beach and anchor containing; LRBA; MGC72098; vesicle trafficking, beach and anchor containing Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 320 SOURCE: Rabbit