CST, 86630S, IDO (D5J4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IDO. Validated for WB,IP,IHC,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IDO (D5J4E) Rabbit Monoclonal Antibody (CST #86630) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 91473 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IDO (D5J4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total IDO (IDO-1, INDO) protein. This antibody does not cross-react with IDO-2 (INDOL1). Some nonspecific staining of normal breast epithelium has been observed. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human IDO protein. Background INDO/IDO1/indoleamine 2,3-dioxygenase (IDO) is an IFN-γ-inducible enzyme that catalyzes the rate-limiting step of tryptophan degradation (1). IDO is upregulated in many tumors and in dendritic cells in tumor-draining lymph nodes. Elevated tryptophan catabolism in these cells leads to tryptophan starvation of T cells, limiting T cell proliferation and activation (2). Therefore, IDO is considered an immunosuppressive molecule, and research studies have shown that upregulation of IDO is a mechanism of cancer immune evasion (3). The gastrointestinal stromal tumor drug, imatinib, was found to act, in part, by reducing IDO expression, resulting in increased CD8 T cell activation and induction of apoptosis in regulatory T cells (4). In addition to its enzymatic activity, IDO was recently shown to have signaling capability through an immunoreceptor tyrosine-based inhibitory motif (ITIM) that is phosphorylated by Fyn in response to TGF-β. This leads to recruitment of SHP-1 and activation of the noncanonical NF-κB pathway (5). Alternate Names I23O1; IDO; IDO-1; IDO1; INDO; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; Indoleamine 2,3-dioxygenase 1; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG