CST, 86641S, CTGF (D8Z8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTGF. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CTGF (D8Z8U) Rabbit Monoclonal Antibody (CST #86641) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92687 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CTGF (D8Z8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total CTGF protein. By Western blot analysis, this antibody detects a 120 kDa protein of unknown identity in both CTGF-positive and CTGF-negative cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val174 of human CTGF protein. Background Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1,3-5). The gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8,9). Alternate Names CCN family member 2; CCN2; Cellular communication network factor 2; Connective tissue growth factor; CTGF; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8; Insulin-like growth factor-binding protein 8; MGC102839; NOV2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG