CST, 8664S, Phospho-SGTA (Ser305) (D23E10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SGTA (Ser305) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SGTA (Ser305) (D23E10) Rabbit Monoclonal Antibody (CST #8664) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-SGTA (Ser305) (D23E10) Rabbit Monoclonal Antibody recognizes endogenous levels of SGTA protein only when phosphorylated at Ser305. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser305 of human SGTA protein. Background SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6). Phosphorylation of SGTA at Ser305 was identified at Cell Signaling Technology (CST) using PhosphoScan , a CST™ LC-MS/MS platform for phosphorylation site discovery (7). Site-specific mutation analysis indicated that phosphorylation at Ser305 is essential for PDGFR α stabilization and PDGFR α-dependent cancer cell survival (7). Alternate Names Alpha-SGT; alphaSGT; hSGT; protein containing three tetratricopeptide repeats; SGT; SGT1; SGTA; small glutamine rich tetratricopeptide repeat containing alpha; small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; UBP; Vpu-binding protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 34 Source/Isotype: Rabbit IgG