CST, 86665T, CNOT7 (D3M6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CNOT7. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CNOT7 (D3M6O) Rabbit Monoclonal Antibody (CST #86665) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CNOT7 (D3M6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CNOT7 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val30 of human CNOT7 protein. Background The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3). Alternate Names BTG1 binding factor 1; BTG1-binding factor 1; CAF-1; CAF1; Caf1a; carbon catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7; CCR4-NOT transcription complex, subunit 7; CNOT7; hCAF-1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG