CST, 86781T, CD133 (D4W4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD133. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD133 (D4W4N) Rabbit Monoclonal Antibody (CST #86781) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:700 Immunohistochemistry (Paraffin): 1:700 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 69914 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD133 (D4W4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD133 protein. The antibody is not sensitive to the glycosylation status of CD133. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the first extracellular domain of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 257-281, which includes a single N-linked glycosylation site (Asn274). Background CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8). Alternate Names AC133; Antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; PROM1; prominin 1; Prominin-1; prominin-like 1; Prominin-like protein 1; PROML1; RP41; STGD4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 133 Source/Isotype: Rabbit IgG