CST, 8681T, ALPP (3E5C7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ALPP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ALPP (3E5C7) Mouse Monoclonal Antibody (CST #8681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ALPP (3E5C7) Mouse Monoclonal Antibody recognizes endogenous levels of total ALPP protein. This antibody may cross-react with a protein of unknown origin at ~140 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human ALPP protein. Background Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor (3). It is expressed primarily in the placenta (4) and may be involved in transplacental IgG transport (5). ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells (6), and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer (7). Alternate Names Alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; alkaline phosphatase, placental (Regan isozyme); Alkaline phosphatase, placental type; alkaline phosphomonoesterase; ALP; ALPI; ALPP; FLJ61142; glycerophosphatase; IAP; Intestinal alkaline phosphatase; Intestinal-type alkaline phosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; PPB1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Mouse IgG2b