CST, 86825SF, CD36 (E8B7S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD36 mouse. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, CD36 (E8B7S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86825) is ready to ship. Product Usage Information This product is the carrier free version of product #28109. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 28109 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD36 (E8B7S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD36 protein and reacts with an epitope surrounding Ile410. Non-specific nucleolar background may be observed by immunofluorescence in methanol fixed samples. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD36 recombinant protein. Background CD36 is a class B scavenger receptor composed of short amino-terminal and carboxy-terminal cytoplasmic domains, two transmembrane domains, and a large glycosylated extracellular domain (1-4). The CD36 receptor has many diverse ligands and cellular functions and is expressed by multiple cell types, including monocytes, macrophages, platelets, endothelial cells, adipocytes, and some epithelial cells (1). Binding of thrombospondin-1 (TSP-1) to CD36 facilitates the inhibition of angiogenesis by TSP-1 (5). CD36 also binds lipids and enables their transport into cells (6). In macrophages, CD36 acts as a receptor for oxidized LDL (Ox-LDL) and is responsible for Ox-LDL internalization, which contributes to development of atherosclerosis (7). The CD36 receptor participates in the innate immune response by acting as a pattern recognition receptor for lipid components of bacterial cell walls and fungal beta-glucans (8,9). CD36 likely influences signaling by interacting with other cell surface receptors including TLRs, integrins, and tetraspanins (8,10,11). Phorbol 12-myristate 13-acetate (PMA)/ 12-O-tetradecanoylphorbol-13-acetate (TPA) induces CD36 expression in the THP-1 monocyte cell line (12). Alternate Names Cd36; CD36 antigen; CD36 molecule; FAT; fatty acid translocase; glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; platelet glycoprotein IV; Q08857; Sca; Scarb3 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 70-110 Source/Isotype: Rabbit IgG