CST, 86846S, Lamin A (133A2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Lamin A/C (full-length). Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Lamin A (133A2) Mouse Monoclonal Antibody (CST #86846) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Immunocytochemistry): 1:12800 - 1:25600 Storage Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Lamin A (133A2) Mouse Monoclonal Antibody recognizes endogenous levels of total lamin A protein. This antibody also reacts with large fragments of lamin A (50 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamin C. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human lamin A protein. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6). Alternate Names 70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C; lamin A/C-like 1; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; Prelamin-A/C; PRO1; Renal carcinoma antigen NY-REN-32 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 74 Source/Isotype: Mouse IgG3