CST, 86881SF, PLC gamma2 (E5U4T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PLCg2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PLC gamma2 (E5U4T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86881) is ready to ship. Product Usage Information This product is the carrier free version of product #55512. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 55512 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PLC gamma2 (E5U4T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PLCγ2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PLCγ2 protein. Background Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP ) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP ) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). Alternate Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; APLAID; FCAS3; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; Phospholipase C-gamma-2; Phospholipase C-IV; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); PLC-gamma-2; PLC-IV; PLCG2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG