CST, 8688T, UBA2 (D15C11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBA2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UBA2 (D15C11) Rabbit Monoclonal Antibody (CST #8688) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBA2 (D15C11) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBA2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln421 of human UBA2 protein. Background The process of SUMO conjugation to target proteins is similar to the molecular chain of events observed with ubiquitin (1). SUMO is conjugated to target proteins through the coordinated action of the cellular SUMO conjugation machinery consisting of E1, E2, and E3 enzymes (2). The canonical SUMO E1 activating enzyme is a heterodimer consisting of SAE1 (AOS1) and UBA2 (SAE2) subunits. Mature SUMO is activated by E1 in an ATP-dependent reaction that generates adenylated SUMO, which functions as a high-energy intermediate in the formation of a thioester linkage between SUMO and Cys173 of UBA2 (3,4). SUMO is subsequently transferred from UBA2 to the SUMO E2 conjugating enzyme, UBC9 (5). Recent evidence suggests that redox regulation of UBA2 serves as a physiologic mechanism to modulate the cellular level of sumoylated target proteins (6). Alternate Names Anthracycline-associated resistance ARX; ARX; FLJ13058; HRIHFB2115; SAE2; SUMO-1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; UBA2; UBA2, ubiquitin-activating enzyme E1 homolog; ubiquitin like modifier activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; ubiquitin-like modifier activating enzyme 2; Ubiquitin-like modifier-activating enzyme 2; UBLE1B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG