CST, 86903S, COL5A1 (E6U9W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COL5A1. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, COL5A1 (E6U9W) Rabbit Monoclonal Antibody (CST #86903) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 80085 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity COL5A1 (E6U9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL5A1 protein. Nonspecific staining was observed in skeletal muscle by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro364 of human COL5A1 protein. Background The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: the fibrillar collagens, the non-fibril forming collagens, and the fibril-associated collagens. These sub groups differ in their structure and supramolecular assembly (3). COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage, and skin (4,5). Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others (6-10). Alternate Names alpha 1 type V collagen; CO5A1; COL5A1; Collagen alpha-1(V) chain; collagen type V alpha 1 chain; collagen, type V, alpha 1; EDSC; EDSCL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG