CST, 86952SF, PKC theta (E1I7Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PKCtheta. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, PKC theta (E1I7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86952) is ready to ship. Product Usage Information This product is the carrier free version of product #13643. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13643 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PKC theta (E1I7Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PKCθ protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro632 of human PKCθ protein. Background Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7). PKCθ is a novel protein kinase C that is predominantly expressed in T cells (8). Recruitment of PKCθ to the immunological synapse following T cell receptor stimulation plays an important role in the activation and proliferation of conventional T cells (9). Conversely, PKCθ negatively regulates the suppressive function of regulatory T cells and is excluded from regulatory T cell immunological synapses (10). Alternate Names KPCT; MGC126514; MGC141919; nPKC-theta; PKC theta; PKC-theta; PKCQ; PKCT; PKCtheta; PRKCQ; PRKCT; protein kinase C theta; Protein kinase C theta type; protein kinase C, theta Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG