CST, 86960SF, TRAF1 (45D3) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TRAF1. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, TRAF1 (45D3) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #86960) is ready to ship. Product Usage Information This product is the carrier free version of product #4715. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4715 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TRAF1 (45D3) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total TRAF1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 57 of human TRAF1. Background TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. Alternate Names EBI6; Epstein-Bar virus-induced protein 6; Epstein-Barr virus-induced protein 6; MGC:10353; TNF receptor associated factor 1; TNF receptor-associated factor 1; TRAF1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit