CST, 8699S, Phospho-MOB1 (Thr35) (D2F10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MOB1A (Thr35) phosphate/MOB1B (Thr35) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-MOB1 (Thr35) (D2F10) Rabbit Monoclonal Antibody (CST #8699) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-MOB1 (Thr35) (D2F10) Rabbit Monoclonal Antibody recognizes endogenous levels of MOB1 protein only when phosphorylated at Thr35. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr35 of human MOB1 protein. Background MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1A and MOB1B, that are encoded by two different genes but which have greater than 95% amino acid sequence identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues (e.g., Thr12, Thr35) by MST1 and/or MST2 (5,10). Phosphorylation at Thr35 by MST1/2 stabilizes MOB1, enhancing its binding and regulation of LATS1 (5). The resultant increase in LATS1 kinase activity promotes inhibitory phosphorylation of the transcriptional co-activators YAP and TAZ (11,12), leading to changes in the expression of genes involved in cell cycle progression (13). Alternate Names C2orf6; FLJ10788; FLJ11595; MATS1; MATS2; MGC33910; MOB kinase activator 1A; MOB kinase activator 1B; MOB1; Mob1 alpha; Mob1 homolog 1A; Mob1 homolog 1B; MOB1 Mps One Binder homolog A; MOB1 Mps One Binder homolog B; MOB1, Mps One Binder kinase activator-like 1A; MOB1, Mps One Binder kinase activator-like 1A (yeast); MOB1, Mps One Binder kinase activator-like 1B; MOB1, Mps One Binder kinase activator-like 1B (yeast); Mob1A; Mob1B; MOB4A; MOB4B; MOBK1B; MOBKL1A; MOBKL1B; MOL1A; MOL1B; Mps one binder kinase activator-like 1A; Mps one binder kinase activator-like 1B; Protein Mob4A; Protein Mob4B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG