CST, 87109S, RNASEH2B (F5W8X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RNASEH2B. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, RNASEH2B (F5W8X) Rabbit Monoclonal Antibody (CST #87109) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RNASEH2B (F5W8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total RNASEH2B protein. This antibody does not cross-react with RNASEH2A protein. This antibody detects a 30 kDa band of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val303 of human RNASEH2B protein. Background The RNase H2 complex forms as a heterotrimer composed of RNASEH2A, RNASEH2B, and RNASEH2C. RNASEH2A is the catalytic component and is well conserved across species, while RNASEH2B and RNASEH2C are quite divergent (1). The RNase H2 complex is responsible for resolving DNA/RNA hybrids, known as R-loops, which can occur during DNA replication and repair (1,2). The C-terminus of RNASEH2B is bound to PCNA, which then guides the complex to nuclear replication foci to resolve misincorporated ribonucleotides (2). Replicative stress leads to the increased expression of RNase H2 subunits, thereby reducing replication fork stalling in response to chemotherapeutics (3). Mutations in RNase H2 subunits cause Aicardi-Goutieres syndrome (AGS), a rare form of encephalopathy characterized by an increased innate immune response triggered by unresolved R-loops, mimicking a viral infection (4-6). RNASEH2B is frequently lost along with RB1 in advanced prostate cancer, which sensitizes cells to PARP and ATR inhibitors (7,8). Alternate Names AGS2; Aicardi-Goutieres syndrome 2 protein; deleted in leukemia 8 protein; Deleted in lymphocytic leukemia 8; DLEU8; FLJ11712; Ribonuclease H2 subunit B; ribonuclease H2, subunit B; Ribonuclease HI subunit B; RNase H2 subunit B; RNASEH2B; RNH2B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG