CST, 87162T, PHF19 (E8P4D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHF19. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, PHF19 (E8P4D) Rabbit Monoclonal Antibody (CST #87162) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ®  Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity PHF19 (E8P4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHF19 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PHF19 protein. Background PHD finger protein 19 (PHF19), also known as polycomb-like protein 3 (PCL3), is a polycomb group protein that functions as an accessory subunit of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at Lys27 by the EZH2 methyltransferase (1). PHF19 recruits PRC2 to target genes by binding trimethylated histone H3 Lys36, a mark of active chromatin, via its Tudor domain (2-4). PHF19 associates with PRC2 and the histone H3 Lys36 demethylases NO66 and FBXL10, and is required to recruit PRC2 and NO66/FBXL10 to stem cell genes during differentiation, resulting in PRC2-mediated trimethylation of histone H3 Lys27, loss of trimethylated histone H3 Lys36, and transcriptional silencing (2-4). Thus, PHF19 is critical for the proper transition of stem cell genes from the active to inactive state during differentiation of embryonic stem cells. Alternate Names hPCL3; MGC131698; MGC149712; MGC149713; MGC23929; MTF2L1; PCL3; PHD finger protein 19; PHF19; polycomb like 3; polycomb-like 3; Polycomb-like protein 3; TDRD19B; tudor domain containing 19B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG