CST, 8721S, UBE2L3 (D5G1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBE2L3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UBE2L3 (D5G1) Rabbit Monoclonal Antibody (CST #8721) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBE2L3 (D5G1) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBE2L3 protein. This antibody does not cross-react with UBE2L6/UBCH8. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu125 of human UBE2L3 protein. Background Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). UBE2L3, also commonly referred to as UBCH7, is a ubiquitin-conjugating enzyme that belongs to a family of four related human genes, It appears as though is the only member of this gene family to be transcribed and translated and that and exist as pseudogenes (2,3). As a ubiquitin-conjugating enzyme, UBE2L3 has been linked to the ubiquitination of numerous substrates via its interaction with protein-ubiquitin E3 ligases such as NEDD4 (4), E6AP (5), Parkin (6), c-Cbl (7), and Triad1 (8,9). There is also evidence suggesting that UBE2L3 can modulate the transcriptional activity of numerous members of the nuclear hormone receptor superfamily such as the glucocorticoid receptor, progesterone receptor, androgen receptor, and retinoic acid receptors (10). Furthermore, UBE2L3 protein levels appear to be regulated by the ubiquitin-proteasome pathway allowing for it to exert control over S-phase entry and progression (11). Alternate Names E2 ubiquitin-conjugating enzyme L3; E2-F1; L-UBC; UB2L3; UBCE7; UBCH7; UbcM4; UBE2L3; Ubiquitin carrier protein L3; ubiquitin conjugating enzyme E2 L3; ubiquitin conjugating enzyme E2L 3; Ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG