CST, 87220S, LAG3 (D2G4O) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying Lag3. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, LAG3 (D2G4O) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #87220) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity LAG3 (D2G4O) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein. Background Lymphocyte activation gene 3 (LAG3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4 T cells, CD8 T cells, Tregs, and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8 T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6). Alternate Names CD223; FDC; LAG-3; LAG3; lymphocyte activating 3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC; Secreted lymphocyte activation gene 3 protein; sLAG-3 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG