CST, 8729S, SUV39H1 (D11B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SUV39H1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SUV39H1 (D11B6) Rabbit Monoclonal Antibody (CST #8729) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SUV39H1 (D11B6) Rabbit Monoclonal Antibody recognizes endogenous levels of total SUV39H1 protein. This antibody does not cross-react with the SUV39H2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp380 of human SUV39H1 protein. Background Human histone methyltransferase SUV39H1 is the homolog of the dominant Drosophila PEV modifier Su(var)3-9 and is composed of 412 amino acid residues (1). It combines two of the most evolutionarily conserved domains of the "chromatin regulators": the chromo and SET domains (2,3). The 60 amino acid chromo domain represents an ancient histone-like fold that directs heterochromatic localizations. It has been demonstrated that the 130 amino acid SET domain contains the methyltransferase catalytic motif, which cooperates with the adjacent cysteine-rich regions to confer histone methyltransferase activity (1). This enzyme selectively methylates histone H3 on Lys9, which generates a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in both gene silencing and supra-nucleosomal chromatin structure (4,5). SUV39H1 histone methyltransferase plays an important role in modification of histone amino termini and regulation of gene expression. Alternate Names H3-K9-HMTase 1; Histone H3-K9 methyltransferase 1; Histone-lysine N-methyltransferase SUV39H1; histone-lysine N-methyltransferase, H3 lysine-9 specific 1; KMT1A; Lysine N-methyltransferase 1A; MG44; Position-effect variegation 3-9 homolog; Su(var)3-9 homolog 1; Suppressor of variegation 3-9 homolog 1; suppressor of variegation 3-9 homolog 1 (Drosophila); SUV39H; SUV39H1; SUV91 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG