CST, 87340S, RBM22 (E9E6Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBM22. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM22 (E9E6Z) Rabbit Monoclonal Antibody (CST #87340) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity RBM22 (E9E6Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM22 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln13 of human RBM22 protein. Background RBM22, known as Cwc2 in yeast, is an RNA-binding protein initially discovered to play a role in calcium signal by binding to ALG-2 (1). RBM22 helps form an active conformation of spliceosome catalytic RNA elements such as the U6 internal stem-loop and the U12-U6 snRNA complexes (2,3). RBM22 helps control various methods of alternative splicing, as knockdown induces both exon skipping and intron retention (4). RBM22 has also been shown to bind DNA and is implicated in transcriptional regulation (5). Cell cycle and differentiation have also been shown to be affected by RBM22 deficiency, as loss of RBM22 causes cytokinesis defects and impaired erythroid differentiation in hematopoietic stem cells (6,7). RBM22 affects the growth and survival of glioblastoma and leukemia (8,9). Alternate Names Cwc2; FLJ10290; fSAP47; functional spliceosome-associated protein 47; Pre-mRNA-splicing factor RBM22; RBM22; RNA binding motif protein 22; RNA-binding motif protein 22; ZC3H16; Zinc finger CCCH domain-containing protein 16 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG