CST, 87361SF, LIN28A (D1A1A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LIN28A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, LIN28A (D1A1A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #87361) is ready to ship. Product Usage Information This product is the carrier free version of product #8641. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8641 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LIN28A (D1A1A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LIN28A protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro201 of human LIN28A protein. Background LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). Alternate Names CSDD1; FLJ12457; LIN-28; lin-28 homolog A; lin-28 homolog A (C. elegans); Lin-28A; LIN28; LIN28A; LN28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; ZCCHC1; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG