CST, 87416T, CRELD1 (E1B6T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRELD1. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CRELD1 (E1B6T) Rabbit Monoclonal Antibody (CST #87416) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Live) Specificity / Sensitivity CRELD1 (E1B6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRELD1 protein. Species reactivity for flow cytometry is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CRELD1 protein. Background Cysteine-rich with EGF-like domain protein 1 (CRELD1), also known as cirrin, is a multi-pass membrane protein in the CRELD family of ER-resident proteins (1-3). During embryonic development, CRELD1 is critical to heart development, and mutations in are associated with atrioventricular septal defect 2 (AVSD2), a common cardiovascular malformation (2-4). CRELD1 may also play a role in homeostasis of the immune system, as low CRELD1 expression was found to be associated with reduced T cell numbers in blood (5). Alternate Names AVSD2; CIRRIN; CREL1; CRELD1; cysteine rich with EGF like domains 1; Cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213; Protein disulfide isomerase CRELD1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG