CST, 87459S, YTHDC1 (P561) Antibody

Polyclonal Antibody for studying YT521. Validated for Western Blotting. Highly specific and rigorously validated in-house, YTHDC1 (P561) Antibody (CST #87459) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity YTHDC1 (P561) Antibody recognizes endogenous levels of total YTHDC1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro561 of human YTHDC1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background YTH domain-containing protein 1 (YTHDC1) and YTH domain-containing protein 2 (YTHDC2) both belong to a family of proteins that bind to RNA. YTHDC1 and YTHDC2 both recognize and bind to N6-methyladenosine(m6A)-containing RNAs; binding is mediated through the YTH domains (1-3). m6A is a modification that is present at internal sites of mRNAs and some non-coding RNAs and plays a role in regulating mRNA splicing, processing, and stability. YTHDC1, also known as splicing factor YT521, regulates alternative splicing by functioning as a key regulator of exon-inclusion or exon-skipping. YTHDC1 promotes exon-inclusion by recruiting pre-mRNA splicing factor SRSF3 to regions containing m6A, while repressing exon-skipping by blocking SRSF10 binding to these same regions (2). Increased expression of YTHDC1 promotes malignant endometrial carcinoma (EC) through alternative splicing of vascular endothelial growth factor A (VEGF-A), resulting in an increase in VEGF-165 isoform and increased EC cell invasion (4). YTHDC2 functions to enhance the translation efficiency of target mRNAs and may play a role in spermatogenesis (5). Alternate Names KIAA1966; Putative splicing factor YT521; Splicing factor YT521; splicing factor YT521-B; YT521; YT521-B; YTDC1; YTH domain containing 1; YTH domain-containing protein 1; YTHDC1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit