CST, 8749S, WFS1 Antibody

Polyclonal Antibody for studying WFS1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, WFS1 Antibody (CST #8749) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WFS1 Antibody recognizes endogenous levels of total WFS1 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala43 of human WFS1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Wolfram syndrome protein (WFS1) is an 890 amino acid protein that contains a cytoplasmic N-terminal domain, followed by nine-transmembrane domains and a luminal C-terminal domain. WFS1 is predominantly localized to the endoplasmic reticulum (ER) (1) and its expression is induced in response to ER stress, partially through transcriptional activation (2,3). Research studies have shown that mutations in the WFS1 gene lead to Wolfram syndrome, an autosomal recessive neurodegenerative disorder defined by young-onset, non-immune, insulin-dependent diabetes mellitus and progressive optic atrophy (4). Alternate Names CTRCT41; FLJ51211; WFRS; WFS; WFS1; WFSL; Wolfram syndrome 1 (wolframin); Wolframin; wolframin ER transmembrane glycoprotein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit